Thermostabilizing mutations
WebbFör 1 dag sedan · Glycogen storage disease type II (Pompe disease: PD) is an autosomal recessively inherited fatal genetic disorder that results from the deficiency of a glycogen hydrolyzing enzyme, acid α-glucosidase encoded by the GAA gene. Here, we describe the molecular basis of genetic defects in an 8-month-old domestic short-haired cat with … WebbThermostabilization can facilitate GPCR structure determination, as has been shown by the structure of the thermostabilized β1-adrenergic receptor (β1AR) mutant, m23-β1AR, which has been thermostabilized in the inactive state. However, it is unclear from the structure how the six thermostabilizing mutations in m23-β1AR affect receptor dynamics.
Thermostabilizing mutations
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Webbför 2 dagar sedan · At the mutation site of Asn372, the WT structure shows that the main-chain carbonyl oxygen of Asn372 forms hydrogen bonds with the side-chain guanidino group of Arg363 (Fig. 5-C). In the N372D structure, the mutation to aspartic acid does not introduce a hydrogen bond between the side-chain carboxylate group of Asp372 and the … Webbas a model membrane protein. Ten single mutations in the extramembrane regions were designed based on a computational prediction of folding free-energy differ-ences upon …
Webb3 sep. 2015 · Mutations conferring new function to a protein often come at the expense of protein folding or stability, reducing overall activity. Over the years, a panel of T7 RNA …
Webb20 apr. 2015 · About 90 thermostabilizing mutations were identified in GPCRs by alanine scanning so far, however, only two of these mutations were predicted to be transferable … WebbIdentification of Thermostabilizing Mutations for Membrane Proteins: Rapid Method Based on Statistical Thermodynamics Journal of Physical Chemistry B 2016 Journal article DOI: 10.1021/acs.jpcb.6b01405 WOSUID: WOS:000375521600005 Contributors : Yasuda, Satoshi; Kajiwara, Yuta; Takamuku, Yuuki; Suzuki, Nanao; Murata, Takeshi; Kinoshita, …
Webb23 dec. 2009 · The mutations resulted in a minor decrease in the Gibbs free energy change of the TcSOD dissociation, while a significant decrease in that of the unfolding of the monomeirc intermediate. These results herein suggested that the additional ion-pairing network at the C- terminus mainly enhanced the structural stability of hyperthermophilic …
Webb28 jan. 2024 · We have developed a methodology for identifying further thermostabilizing mutations for an intrinsically thermostable membrane protein. The methodology … kruss advance softwareWebb19 nov. 2024 · Finally, two mutations, Pro149Cys and His158Cys, showed potential for stabilizing the protein structure as confirmed by a structure-based stability analysis tool. The findings in this study highlight the opportunity of using disulfide bond modification as a new alternative technique to enhance the thermal stability of GOx. krus scholarshipWebb4 sep. 2013 · The first patent concerns the identification of a stabilizing mutation at position 633 of the amino acid sequence of T7 RNAP involving a serine to proline amino acid change. The second deals with three additional … kruss spectroscopeWebb1 mars 2024 · The created thermostable HMFO mutant was further studied as a biocatalyst for the production of FDCA from HMF and was shown to perform significantly better than the original HMFO. Conclusions The described new gene shuffling approach quickly discriminates stable and active multi-site variants. This makes it a very useful … krüss drop shape analyzerWebb本发明提供了一种呼吸运动预测方法，包括如下步骤：构建BP神经网络，并融合模拟退火遗传算法对BP神经网络进行优化；优化BP神经网络的加权误差；获取呼吸运动数据并对呼吸运动数据进行处理；利用处理后的呼吸运动数据对优化加权误差后的BP神经网络进行训练以获取强回归器；利用强回归器对 ... krus scrabbleWebb25 juli 2024 · Although the three-dimensional structures of G-protein-coupled receptors (GPCRs), the largest superfamily of drug targets, have enabled structure-based drug … k - russian dolls on the christmas treeWebbThe G protein-coupled receptors (GPCRs) form a large, physiologically important family of membrane proteins and are currently the most attractive targets for drug discovery. We … krus supply co