Promega gsh gssg
WebThe GSH/GSSG-Glo™ Assay is a luminescence-based system for the detection and quantification of total glutathione (GSH +GSSG), GSSG and GSH/GSSG ratios in cultured … WebGSH+GSSG / GSH Assay Kit (Colorimetric) (ab239709) provides a convenient, colorimetric method for analyzing either total glutathione or the reduced form glutathione alone using a microtiter plate reader. The assay is based on the glutathione recycling system by DTNB and glutathione reductase.
Promega gsh gssg
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WebJun 7, 2024 · One microgram of cDNA was used to detect various spliced forms of Xbp-1 using GoTaq DNA polymerase (#M3001, Promega, Madison, WI, USA). The PCR … WebGSH is easily oxidized to the disulfide dimer GSSG. Because of the use of glutathione reductase in the Cayman GSH assay kit, both GSH and GSSG are measured and the assay reflects total glutathione. The kit can also be used to measure only GSSG by following an alternative protocol.
WebSep 29, 2024 · GSH + GSSG levels and ROS production in LPS-treated cells with or without BSO. Cells were pretreated with 120 µM BSO for 24 h, followed by 10 ng/ml LPS for 2 h. Total GSH + GSSG and GSSG levels measured in the cell lysates are the mean ± SD of six biological replicates from three independent experiments and are expressed as … WebJun 7, 2024 · One microgram of cDNA was used to detect various spliced forms of Xbp-1 using GoTaq DNA polymerase (#M3001, Promega, Madison, WI, USA). The PCR amplification protocol was set to 10 min at 95 °C, followed by 1 min each at 95 °C, 59 °C, and 72 °C for 50 cycles, and final elongation at 72 °C for 10 min. cDNA (5 ng) was used for …
WebThe GSH/GSSG-Glo™ Assay is a luminescence-based system that detects and quantifies total glutathione (GSH + GSSG), GSSG and GSH-to-GSSG ratios in cultured cells. Reduced … WebApr 14, 2024 · I GSH and GSSG levels in HyPer-DAO-NLS wt and C148A cells n.t. or after treatment with 50 mM D-ala or ... Glutathione levels were determined using the GSH/GSSG …
WebThe GSH/GSSG-Glo™ Assay is a luminescence-based system that detects and quantifies total glutathione (GSH + GSSG), GSSG and GSH-to-GSSG ratios in cultured cells. Reduced … GSH/GSSG-Glo™ Assay. Homogeneous assay to quantify total glutathione and … GSH/GSSG-Glo™ Assay. Homogeneous assay to quantify total glutathione and … The ApoLive-Glo™ Multiplex Assay measures both the number of viable cells …
WebAfter treatment of 10 μM NR1 for 72 h, cells (1×10 6 per sample) were lysed in 100 μL of lysis buffer to perform the GSH/GSSG assay. The GSH/GSSG assay was performed using the Green Fluorometric Detection Kit (AAT Bioquest), which uses a proprietary nonfluorescent dye that becomes strongly fluorescent upon reacting with GSH (Ex/Em =490/520 nm). green day let yourself go lyricsWebApr 18, 2024 · Interestingly, while 3T3 KRAS V12 and EN cells exhibited a lower GSH/GSSG ratio relative to 3T3 MSCV cells under ambient conditions, these cells had similar GSH/GSSG ratios as 3T3 MSCV cells following H 2 O 2 treatment ( Fig. 1 F ). green day let yourself go downloadWeb1.1.2 酶和试剂 人源溶菌酶标准品购买于北京经科宏达生物技术有限公司；NdeⅠ和XhoⅠ限制性核酸内切酶购买于日本TaKaRa公司；T4 DNA连接酶购买于美国Promega公司；DNA Marker 5000购买于康为世纪生物科技有限公司；琼脂糖、尿素、DTT、EDTA、Triton X-100、IPTG、PMSF购买于 ... fls instituteWebGSH, GSSG, activities of histone deacetylase (HDAC) and histone acetyltransferase, apoptosis, and phagocytosis were assessed in isolated AMs. fls investment brian hanWebPromega GSH/GSSG-Glo™ Glutathione Assay. The GSH/GSSG-Glo™ Assay is a luminescence-based system for the detection and quantification of total glutathione (GSH … fls intracorporeal knotWebThe simple two-reagent-addition GSH detection assay minimizes the number of assay steps compared to conventional GSH assays and is easily adapted to higher throughput … green day lights outWebMethyl mercury (MeHg) is an organic highly toxic compound that is transported efficiently via the human placenta. Our previous data suggest that MeHg is taken up into placental cells by amino acid transporters while mercury export from placental greenday lighting