Primescript double strand cdna synthesis kit
WebApr 13, 2024 · RNAiso reagent (TaKaRa, Japan) was used to extract the total cellular RNA, and PrimeScript™ RT kit (TaKaRa, Japan) was used to obtain the cDNA. SYBR Premix Ex … WebBrand: Takara Bio. PrimeScript IV 1st strand cDNA Synthesis Mix contains a 5X premix for synthesizing first-strand cDNA from total RNA or poly A+ RNA. A cDNA synthesis reaction …
Primescript double strand cdna synthesis kit
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WebPrimescript ⅱ 1st Strand Cdna Synthesis Kit, supplied by TaKaRa, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, … WebThymocytes were sorted into 96-well plates containing the lysis buffer (0.2% Triton-100, 1U/µl RNase inhibitor)Reverse transmission was performed using SuperScript SLIDE …
Web14. Dry the cDNA at 37°C for 10 min to evaporate residual ethanol, and proceed to Analysis of the cDNA Products. Page 3 Second-Strand Synthesis This protocol is suitable for … Web3) PrimeScript 1st Strand cDNA Synthesis Kit. Excellent elongation: able to synthesize long cDNAs (up to 12 kb) with good yield. Oligo dT and random primers included: use of polyA …
WebLiterature # TM316. The GoScript™ Reverse Transcription System is a convenient kit that includes a reverse transcriptase and an optimized set of reagents for efficient synthesis … WebIrma Pakutinskiene posted on LinkedIn
WebApr 13, 2024 · RNAiso reagent (TaKaRa, Japan) was used to extract the total cellular RNA, and PrimeScript™ RT kit (TaKaRa, Japan) was used to obtain the cDNA. SYBR Premix Ex Taq II real-time PCR kit (TaKaRa, JAPAN) was used to detect the mRNA expression of the gene. GAPDH was set as the internal control. For the miRNAs, Mir-X™ miRNA first-strand …
WebFirst-Strand cDNA Synthesis Kit contains the following reagents, sufficient for up to 55 first-strand syntheses, each of which will produce enough material for up to 15 amplification … trotting club bunburyWebApr 12, 2024 · The total RNA was extracted from grains at different developmental stages using the MiniBEST Plant RNA Extraction Kit (TaKaRa), and the first-strand cDNA was obtained by using the PrimeScript RT reagent Kit with gDNA Eraser (TaKaRa). Quantitative real-time PCR (qRT-PCR) was performed using TB Green Premix Ex Taq II (Tli RNaseH … trotting hill park booksWebApr 8, 2024 · Total RNA was isolated from cells using the Fastagen200 kit (Fastagen, Shanghai, China). Reverse transcription of RNA (1.0 μg) to cDNA was conducted by the PrimeScript RT Reagent Kit (TaKaRa, Osaka, Japan). Real-time PCR was carried out using SYBR Premix Ex Taq TM II (TaKaRa, Osaka, Japan) with primers listed in Supplementary … trotting a horse out by handWebSecond Strand cDNA Synthesis Kit Catalog Number A48570, A48571 Pub. No. MAN0019413 Rev. A.00 Product description Invitrogen™ Second Strand cDNA Synthesis … trotting horse associationWebThe RNA purity and concentration were measured with a NanoDrop TM ND1000 spectrophotometer (NanoDrop Technologies, Rockland, DE, USA). cDNA was synthesized … trotting hall of fameWebAug 11, 2024 · The presently described and disclosed technology includes, in one example, a method, comprising: extracting a sequence of a spike protein of a first virus from a first non-human mammal that is previously exposed to an infection by the first virus; identifying a target antigen specific to the spike protein; and injecting an mRNA therapeutic comprising … trotting horse crossword clueWebThe SureScript™ First-Strand cDNA Synthesis Kit includes a reverse transcriptase and a specialized set of reagents designed to yield cDNA that is optimal for gene cloning, cDNA … trotting horse clip art