Piperidine treatment of dna
WebbNonirradiated DNA duplex submitted to Fpg treatment (lanes 4 and 8) or to piperidine treatment (lanes 7 and 11). For control, uniquely 5′ end-labeled upper strand DNA duplex nonirradiated... Webb1 feb. 1999 · In addition, the piperidine treatments were performed with 0.05 OD of unlabeled modified oligodeoxyribonucleotides. The resulting DNA fragments were desalted by several 3 M ammonium acetate:ethanol (1:3 v/v) precipitations at −20°C, prior to analysis by MALDI-TOF mass spectrometry.
Piperidine treatment of dna
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WebbSpontaneous resistance against the P4Cs emerged at a frequency of 10-8 /CFU and mapped to gyrA and gyrB encoding the subunits of DNA gyrase. Biochemical studies … WebbFree and protein-bound DNA molecules can be resolved in a non-denaturing band-shift gel. Bands are excised and eluted from the gel, and cleaved at the methylated positions with …
Webb9 maj 2024 · Historically, purpurin has been used not only as a textile dye but also as an ingredient of herbal medicine and a food coloring agent. Purpurin has been reported to … Webb5 juni 2003 · After piperidine treatment, the DNA fragment was electrophoresed on an 8% polyacrylamide/8 M urea gel and the autoradiogram was obtained by exposing film to the gel Full size image
Webb29 mars 2024 · However, it is very easy to understand and requires just 4 steps. 1) Preparation of Your Sample The DNA used in Maxam-Gilbert sequencing is first denatured into a single-stranded chain, and labeled … Webb15 jan. 2006 · In addition, the studies indicated that the extended thermal treatment (60 min at 100 °C) or piperidine treatment of DNA alkylated by (+)-duocarmycin SA was sufficient to cleave DNA with clean ...
Webb12 okt. 2024 · Bisulfite treatment of DNA leads to deamination of cytosine to uracil but not of 5mC to thymine, thereby translating a cytosine modification into a change in the sequence of canonical nucleobases...
WebbMetrics Vanadyl sulfate reacts with the peroxy acid oxidant KHSO 5 to produce guanine-selective oxidation of a 167-bp restriction fragment of DNA. The oxidized lesions result in strand scission after hot piperidine treatment. bundle of asphalt shinglesWebb11 nov. 1997 · The base sequence in PNA-1/DNA-1X was specifically designed to probe for the corresponding reaction in PNA/DNA hybrids and to examine its mechanism. DNA-1X … bundle of arrows tattooWebb15 okt. 1992 · Each of the 5'-end labeled ss DNAs was treated with hot piperidine and analyzed on a sequencing gel as in Fig. 2. Lane 1; unmodified 46 mer DNA and lanes 2, 3 and 4; DNAs containing 8-OH-Gua, B-OH-Ade and O6-MeGua, respectively. half of 569Webb7 juli 2009 · Titanium dioxide (TiO 2) is a potential photosensitizer for photodynamic therapy. In this study, the mechanism of DNA damage catalyzed by photo-irradiated TiO … half of 551Webb15 okt. 1992 · The two findings indicate that 8-hydroxyguanine (8-OH-Gua) is a hot piperidine-sensitive lesion in DNA. These are cleavages of DNA containing 8-OH-Gua at … bundle of baby clothesWebb10 aug. 2024 · Furthermore, since the primary lesion is that of a base modification, piperidine treatment, or other weakly basic conditions, are needed to convert the base lesion into a strand-scission event. Another scheme for oxidative cleavage of DNA mediated by metal complexes involves formation of a coordinated ligand radical bound … half of 575Webb17 maj 2024 · This indicates that P4Cs, similar to fluoroquinolones, cause DNA gyrase-mediated DNA damage. Together, our results show that P4Cs present a novel class of mycobacterial DNA gyrase inhibitors... half of 571