WebAug 8, 2024 · Immunoblotting of GBP1 from STm-infected IFNγ-primed macrophages revealed caspase-1 activation and formation of p47 GBP1 from cells expressing WT GBP1 but not GBP1 D192E (Figure 4 E). Together, these results point toward the specificity of caspase-1 in cleaving GBP1, and that neither caspase-4 nor caspase-8 (active during Tg … WebMay 6, 2011 · Macrophages stably expressing doxycycline-repressible Gbp1, Gbp7, or Gbp10 with different N-terminal tags for single-step or two-step capture via tandem affinity purification (TAP) were generated. In addition, glutathione S -transferase (GST)–Gbps were immobilized to ensure sufficient bait for isolating partners from stringent detergent ...
A Family of IFN-γ–Inducible 65-kD GTPases Protects Against
WebJun 3, 2024 · GBP1 is an essential mediator of macrophage cell death during Toxoplasma infection. We investigated the impact of IFNγ‐priming on host cell death in PMA‐differentiated human THP‐1 macrophage‐like cells upon infection with type I (RH) and type II (Pru) Tg, which differ in their virulence effector repertoire (Saeij et al, 2005).We … WebBackground: Mainstream application of cancer immunotherapy is hampered by the low response rate of most cancer patients. A novel immunotherapeutic target or a biomarker predicting response to … the mark of zorro cast
Human GBP1 Differentially Targets - ScienceDirect
WebMay 27, 2024 · 125 macrophages. We further determined that human GBP1 was essential for maximal inflammasome activation and that IFN-g-primed macrophages had a significant increase in GBP1 and GBP2 localization to the LCV compared to unprimed macrophages. GBP1 and GBP2 were recruited to L. pneumophila in a T4SS-dependent manner, … WebJun 3, 2024 · GBP1 targets Toxoplasma and Salmonella in macrophages and regulates host cell death. GBP1 promotes Toxoplasma DNA detection by AIM2-ASC-caspase-8 leading to apoptosis and targeting of Salmonella by caspase-4 leading to pyroptosis.. IFNγ-induced GBP1 translocates to Toxoplasma gondii-and Salmonella Typhimurium … WebGBP1 is an essential mediator of macrophage cell death during Toxoplasma infection We investigated the impact of IFNc-priming on host cell death in PMA-differentiated human … tier list tabaco