2024 Clontech 质粒pegfp-n1

Clontech 质粒pegfp-n1

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August undefined, 2024

WebJun 6, 2015 · required, stable transformants can be selected using G418 (7). pEGFP - N1 can also be used simply to express EGFP. in a cell line of interest (e.g., as a transfection marker). Location of features: • Human cytomegalovirus (CMV) immediate early promoter: 1–589. Enhancer region:59–465; TATA box: 554–560. http://www.synthesisgene.com/vector/pEGFP-C1.pdf

分子克隆之基础篇~一文读懂质粒 - 知乎 - 知乎专栏

Web1. Centrifuge at 5,000×g for 5 min. 2. Carefully open the tube and add 20 μl of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin to concentrate the liquid at the bottom. Speed is less than 5000×g. Web哺乳表达质粒 pEGFP-C1. 哺乳表达质粒 pEGFP-N1. 哺乳表达质粒 pEGFP-MHF. 哺乳表 … small embassy

pEGFP-N1 pEGFPN1载体图谱质粒图谱、序列、价格、抗性、测序 …

WebSep 8, 2016 · 本实验设计计划通过基因工程的方法，利用大肠杆菌表达系统表达增强型绿色荧光蛋白，首先pEGFP-N3和pET-28a (+)质粒从含有相应质粒的的E.coli中提取出来，然后利用双酶切法将两种质粒切开再利用T4连接酶构建pET-28a-EGFP质粒DNA，将重组质粒转入E.coliDH5α中，最后 ... WebpEGFP-N1 (CLONTECH Lab., Palo Alto, CA, USA) has been modified to the dual-cassette expression vectors named pEGFP-Ks by the relocation of its ... MCS in the pEGFP-N1. The pEGFP-K2 vector contains ... WebpEGFP-C1. Vector for fusing EGFP to the N-terminus of a partner protein. For other … small elf ears

Clontech 质粒pegfp-n1

Web哺乳表达质粒 pEGFP-C1. 哺乳表达质粒 pEGFP-N1. 哺乳表达质粒 pEGFP-MHF. 哺乳表达质粒 pDsRed1-N1. 哺乳表达质粒 pmcherry-c1# 哺乳表达质粒 pRI-GFP/hygeo. 哺乳表达质粒 pCXWB-EBNA1. 哺乳表达质粒 pmR-mCHerry. 哺乳表达质粒 pTT5. 哺乳表达质粒 pTagBFP-N vector. 哺乳表达质粒 p3XFLAG-CMV-7.1 WebMay 3, 2006 · Clontech Laboratories, Inc. A Takara Bio Company 1290 Terra Bella Ave. Mountain View, CA 94043 Technical Support (US) E-mail: [email protected] www.clontech.com Vector Information pAcGFP1-N1 Vector Information PT3716-5 Cat. Nos. 632469 632426. Restriction Map and Multiple Cloning Site (MCS) of pAcGFP1-N1 …

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WebpEGFP-N1 Vector Information PT3027-5 GenBank Accession #U55762 Catalog #6085-1 … WebpEGFP-N1和pEGFP-N1-EMP1质粒转染Tb3.1细胞48 h后 GFP的表达荧光显微镜 × 100. …

WebpEGFP-N1. Vector for fusing EGFP to the C-terminus of a partner protein. For other … WebpEGFP-N1 encodes a red-shifted variant of wild-type GFP which has been optimized for brighter fluorescence and higher expression in mammalian cells. (Excitation maximum = 488 nm; emission maximum = 507 nm.) pEGFP-N1 encodes the GFPmut1 variant which contains the double-amino-acid substitution of Phe-64 to Leu and Ser-65 to Thr. The …

WebpEGFP-N1,pEGFP N1载体价格1000元，pEGFPN1载体质粒图谱(Vector map),载体序 … WebpEGFP-N1: Antibiotic Resistance: Kanamycin Length: 4733 bp: Type: Fluorescent …

WebPlasmid pEGFP-C1-FLAG from Dr. Steve Jackson's lab contains the insert FLAG tag and is published in J Cell Biol. 2013 Jul 29. This plasmid is available through Addgene. ... Clontech Backbone size ... pEGFP-N1-FLAG pEGFP-C1-FLAG-Ku80 ...

Web18 rows · Name Vector Type Resistance Marker Bacterial Resistance Source Sequence … small embedded linux computerWebThermo Scientific 分子克隆. 灵活。. 创新。. 快速。. Thermo Scientific CloneJET PCR 克 … small elongated breakfast rollshttp://www.honorgene.com/product_list/carrier_library/123423.html small embedded linux computer no wi-fiWebPlasmid pEGFP-N1-FLAG from Dr. Patrick Calsou's lab is published in Nucleic Acids Res. 2014 Oct 1;42(14):9047-62. doi: 10.1093/nar/gku601. Epub 2014 Jul 16. This plasmid is available through Addgene. ... Clontech Backbone size (bp) 4733 Modifications to backbone. Insertion of a FLAG tag in front of GFP together with an ATG and a strong … small elf picture small elongated marine invertebratehttp://www.bioon.com.cn/protocol/showarticle.asp?newsid=2628&typename=DNA%E7%80%B9%E7%82%BA%E7%8D%99&sortid=1737&typeid=2413 small elite groupWebMar 27, 2024 · 图2b：clontech pegfp-n1载体信息. 但是，在许多情况下，我们需要根据目标蛋白的实际情况修饰ffp，如内质网蛋白和膜蛋白：一个典型的内质网蛋白通常含有两段关键序列（信号序列，ss；内质网滞留序列，kdel），若其功能域靠近c端，fp应置于ss下游2-10个氨基酸处，这样可以提高ss的切割效率，并有利于 ... small elvish decorative jar set with lids