Chlorotrityl resin loading protocol
WebA fast and efficient microwave (MW)-assisted solid-phase peptide synthesis protocol using the 2-chlorotrityl chloride resin and the Fmoc/tBu methodology, has been … WebLoading (determined from the substitution of the Fmoc-Ala-Leu loaded resin): 1.00 - 1.80 mmol/g Swelling Volume (in CH₂Cl₂): 2.0 - 5.0 ml/g Total swelling volume acc. Houben Weyl (in CH 2 Cl 2 ): ≥ 4.2 ml/g The …
Chlorotrityl resin loading protocol
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WebApr 13, 2024 · In recent years, the work of various research groups has allowed the substitution of the hazardous solvents most widely used in solid-phase peptide synthesis, namely DMF, NMP, DCM, DEE, among others, … WebFirst, we loaded 2-chlorotrityl chloride resin for carboxylated peptides. The first amino acid was loaded manually by treating 1 g of 2-chlorotrityl chloride resin (1.6 mmol/g of Cl) swollen in DCM with a solution of 0.6 mmol amino acid and 1 mL of iPr 2 Net in 5 mL of DCM for 1 h, followed by adding 5 mL of MeOH and agitating for an additional ...
WebThe functionalization degree with reactive chlorotrityl chloride linker was 1.55 mmol/g. For loading of the related trityl chloride resin (0.8 mmol/g), Hall et al. employed 50 equivalents of polyamine, which were added in four portions as solution in CH 2 Cl 2 resulting in 0.73 mmol/g of resin-bound 1,3-diaminopropane. Considering the almost ...
WebDec 27, 2024 · Common protocols for the loading of 2-CTC and MBH-Br resins presume the application of DCM, THF, or DMF; these solvents are considered as undesirable in … WebYou can bring it back to activated chloro resin by following this activation protocol: Suspend the resin in DCM (approximately 10 mL per gram of resin). Cool the …
WebMay 6, 2014 · Synthesis of Fmoc hydrazine 2CTC resin 2-Chlorotrityl chloride resin (loading=0.57 mmol/g) (2 g) was swollen in CH 2 Cl 2 (16 mL) at 0 °C. Fmoc hydrazine hydrochloride (1.16 g, 4 equiv) and diisopropylethylamine (2.02 mL, 10 equiv) were dissolved in DMF (20 mL) and CH 2 Cl 2 (4 mL).
WebA process for the preparation of solid phase bonded 2-chlorotrityl chloride (2-CTC resin) of formula I wherein Ps is a polymeric support and n has the following meaning: 1≧n≧0 comprising the... gym rat\\u0027s development nyt crosswordWebLoading the Resin To a 100mL peptide synthesis vessel, add 2-chlorotrityl chloride (CTC) resin (1.1 mmol/g, 0.360 g, 0.400 mmol). Add 20 mL DMF and allow them to swell for 30 min under N 2. Drain the beads under vacuum and add 10 mL DMF. Add 500 mg Fmoc-Ala-OH (1.60 mmol) and 2.5 mL i- Pr 2 EtN, and mix under N 2 for 15 min. bpc 157 kneeWebProcedure for Loading Fmoc-Amino Acid to 2-Chlorotrityl Chloride Resin Weigh 10 g 2-chlorotrityl chloride resin (15 mmol) [1 g, AnaSpec Catalog # 22229; 5 g, AnaSpec … gym rat\u0027s development nyt crosswordWebA method of preparing a phosphoramidate morpholino oligomer (PMO), the method comprising: coupling a solid support, morpholino subunit monomer or an oligomer thereof, with a monomer of Formula (I) having the structure: wherein: R 1 is a protected or unprotected hydroxyl group, a chlorophosphoramidate group, a phosphoramidate linkage … gym rat\\u0027s development crossword clueWebOct 2, 2024 · Add a solution of first protected amino acid Fmoc-AA-OH (1.0 eq for a resin load of 0.6–0.8 mmol/g) and DIPEA (3 eq., 1.2 mmol) in DCM to the resin ( see Note 5) and shake it for 10 min. Add 7.0 additional equivalents of DIPEA (2.8 mmol) and shake for one more hour at room temperature. 4. gym rat\u0027s pride crosswordWebPrep time: 30 mins Reaction time: 8–24 hours Scheme 1: Loading 2-chlorotrityl chloride resin. Resin is drawn as carbocation, whereas amino acid is drawn as carboxylate, to ease explanation of chemistry. 1. Weigh out appropriate amount of resin. Generally we use 300 mg for a 0.1 mmol scale synthesis. gym rat\\u0027s development ny times crosswordWebThese are recommended general procedures for preparing peptides by solid phase methods utilizing AAPPTec products. Peptide Synthesis Resins, Loading and Cleavage Procedures 1035 – Aminomethyl Resin 1039 – Carboxypolystyrene 1027 – 2-Chlorotritylchloride Resin for Protected Peptide Synthesis gym rat\\u0027s pride crossword